Core Facilities

The four state-of-the-art Facilities Cores provide Center members with the technologies, expertise and services that are not available from other sources at MIT. The following Facilities Cores reflect the new research directions of the Center:

Animal Models Facilities Core
Contact
Director: Prof. James Fox

The Animal Models Facilities Core, directed by Professor James Fox of the Division of Comparative Medicine and the Department of Biological Engineering, provides Center members with the latest technology for the application of animal models to environmental health research. Center members are provided with an extensive array of services related to animal models and pathology.

The objective of the Animal Models Facilities Core is to provide Center members with state-of-the-art pathology and imaging support, transgenic resources and a centrally-manged AAALAC-approved animal holding and surgical facility. The Core is staffed with experienced personnel and is equipped with essential equipment to generate genetically engineered mice, cryopreserve sperm and embryos, rederive imported mice by embryo derivation, provide colony management, prepare and interpret tissue samples by histological and image analysis. Read More

If you would like to use this facility, please complete the request form.

Bioimaging and Chemical Analysis Facilities Core
Contact
Dr. Robert Croy
Dr. Michael DeMott
Dr. Stephen Slocum

This Facilities Core is temporarily overseen by interim co-Directors Drs. Robert Croy, Michael DeMott and Stephen Slocum.  The Bioimaging and Chemical Analysis Facilities Core is a central resource in analytical chemistry, separation science, metabolomics, and proteomics for Center members, and provides research participants with analytical expertise, training, and access to state-of-the-art instrumentation. This Facilities Core is home to a world-class chromatography and mass spectrometry facility, and provides CEHS members with state-of-the-art chemical molecular separation, identification, and quantification capabilities, along with consultation for training, experimental design, and data interpretation. The mass spectrometry laboratory is located in Building 16 (room 720). The imaging laboratory is located in Building 16 (room 318).  Read More

If you would like to use this facility, please complete the request form.

Genomics Facilities Core
Contact
Director: Dr. Stuart Levine

The Genomics Facilities Core, led by Dr. Stuart Levine, provides CEHS researchers with access to state of the art tools, services and expertise in the areas of genomics, systems biology, bioinformatics, and BioIT. These tools provide researchers a broad spectrum of technologies to understand cell state and how environmental stresses result in perturbations of the cellular systems and human health. The core services are available either through walkup or assisted services and training is available in all areas. Read More

If you would like use this facility, please complete the request form or email biomicro@mit.edu.

Integrative Health Sciences Facilities Core
Contact
Co-Directors: Prof. Michael Yaffe and Prof. James Fox

The CEHS research portfolio extends from basic studies of the physics and chemistry of our environment, to clinical studies of how exposure impacts human health and, then ultimately to policy recommendations that protect and improve public health and welfare. The Integrative Health Sciences Facilities Core (IHSFC) enhances the impact of laboratory research by lowering the barriers to clinical translation of basic studies into new clinical practice.  In doing so, the IHSFC amplifies the impact of the CEHS by helping to deliver our science and engineering advances to help people. The strength of the IHSFC lays in its leadership, which is comprised of basic researchers, leaders in technology development, animal researchers, clinicians, and an epidemiologist. While a great strength of the IHSFC is its clinical connection and support, it is important to note that the IHSFC facilitates translations at all levels from the most basic research, to animal and human studies and ultimately to work that impacts policy. Read More

If you would like use this facility, please complete the request form.

ADVANCED ANALYTICAL FEMTO PULSE
Contact

The Genomics Facilities Core located in Building 68-316 recently purchased (May 2017) a new fragment analysis instrument from AATI.  The FEMTO Pulse is a brand new instrument that provides a remarkable upgrade over the existing AATI Fragment Analyzer and Agilent BioAnalyzer technologies that have been available for many years.  While still an instrument to analyze the lengths of nucleic acids, the FEMTO Pulse adds a pulse filed generator to allow the separation and characterization of significantly longer molecules, out to 200,000 base pairs.  

165 Kb ladder separated on the FEMTO Pulse Automated Pulsed-Field CE Instrument using the Ultra-Short Capillary Array (22-40). Image from AATI.

Additionally, the FEMTO pulse has significantly improved optics that allow several orders of magnitude of additional sensitivity.  Where the AATI fragment analyzer bottoms out a sensitivity of 2-3pg/ul, the FEMTO pulse has nearly 1000x improved sensitivity which may enable this instrument to directly observe molecular reactions without the need for amplification or modification with radioactive nucleotides.

 

Serial dilution of 300bp fragment on the FEMTO pulse. Figure from AATI

For additional information of the Femto Pulse instrumentation, please contact Dr. Stuart Levine (slevine@mit.edu).