Bioimaging and Chemical Analysis Facilities Core

Contact: Dr. Robert Croy, Michael DeMott, and Stephen Slocum

The Bioimaging and Chemical Analysis Facilities Core provides Center members with state-of-the-art tools and techniques for the characterization and quantification of chemical substances and modifications of cellular compounds ranging from small molecules, e.g., metabolites and reaction products, to nucleic acid oligomers and intact proteins. The Core resources also include a comprehensive suite of data-handling software for qualitative and quantitative analyses, database searching, bioinformatics, pathway analysis, statistics and graphics. As a resource for CEHS members, the Core allows researchers to use the facilities at three levels: fully trained users, supervised analyses, and as a service.

MAJOR INSTRUMENTS

DATA ANALYSIS

  • Bioinformatics (Database searching, pathway analysis)
  • Quantitation (Spectral counting, iTRAQ, multiple reaction monitoring)
  • Custom software (Deconvolution, unique protein/peptide modifications)
  • Statistics (Regression analysis, principal component analysis, clustering)

IMAGING

  • Plate reader (varioskan)
  • Scanning Cytometer (Compucyte)
  • Flow Cytometry (Accuri C6)
  • Phosphoimager (Typhoon)
  • High throughput Imaging (Cellomics)